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. 2018 May 29;12:174–183. doi: 10.1016/j.omtn.2018.05.004

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

uc.454 Regulated Expression of HSPA12B

(A) HSPA12B mRNA expression was assessed by real-time qPCR after uc.454 was upregulated and downregulated in LC cell lines (NCI-H2170 and SPC-A-1). (B) After over (down)-expression of uc.454 in NCI-H2170 and SPC-A-1 cells, western blotting analysis was used to determine HSPA12B protein levels. (C) After uc.454 overexpression in NCI-H2170 and SPC-A-1 cell lines, immunofluorescence assay was used to detect protein expression levels of HSPA12B. (D) RIP results showed that uc.454 interacted with transcriptional activator HSPA12B. The agarose gel electrophoresis graph showed the PCR product levels of RIP. Error bars represent mean ± SD of triplicate experiments.