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. 2018 May 29;12:174–183. doi: 10.1016/j.omtn.2018.05.004

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

HSPA12B Depletion Induced Apoptosis by Regulating Expressions of Bcl-2 Protein Family Members

(A) The mRNA levels of HSPA12B were downregulated in NCI-H2170 and SPC-A-1 lung cell lines after treatment with two siRNAs against HSPA12B. (B) After siRNA transfections, CCK-8 assays were performed in NCI-H2170 and SPC-A-1 cells. (C and D) The colony formation assays were performed to assess LC cell proliferation in NCI-H2170 cell lines. (D) Flow cytometric analysis showed that down-expression of HSPA12B could induce cell apoptosis. (E) After HSPA12B siRNA transfection in NCI-H2170, protein levels of HSPA12B-activated Caspase-3 and Bcl-2 family proteins (Bax and Bcl-2) were detected by western blotting. The β-actin protein was used as an internal control. *p < 0.05.