Figure 3.

PCR temperature consistency. (a) Thermal cycling profile as measured by thermistors within the heat block (dashed line) and embedded within a test cartridge PCR well (solid line). PCR assays demonstrated here used a 2-minute hot-start at 95 °C followed by 45 cycles of 20 seconds at 65 °C anneal and 5 seconds at 95 °C denature. (b) Temperature ramp profile demonstrating close agreement between the targeted temperature with the actual measured temperature in the cartridge with error measuring <1 °C throughout the relevant range of temperatures for melt analysis of amplified nucleic acid products.