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. 2018 Jun 28;9:2523. doi: 10.1038/s41467-018-04952-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The OsGSK2-phosphorylated CYC U2 interacts with D3. a CYC U2 protein levels in the Ni/CYC U2-FLAG and CYC U2-FLAG/OsGSK2-OX F₁ hybrids lines. The wild-type Nipponbare crossed with CYC U2-FLAG as a control. The mesocotyls are pre-treated with cycloheximide (CHX) for 3 h. Anti-FLAG was used to detect CYC U2-FLAG fusion protein level, and anti-tubulin was used for equal loading. b Interactions between CYC U2 and D3 in BiFC assays. Scale bar, 100 µm. c Interaction between CYC U2 and D3 in the Co-IP assays. The proteins were expressed and extracted from tobacco leaf and immunoprecipitated by anti-FLAG M2 magnetic beads. Gel blots were probed with anti-FLAG or anti-MYC antibody. d D3 interacts with the OsGSK2-phosphorylated CYC U2 in GST pull-down assays. The kinase dead type, OsGSK2^K69R-HIS, is as the negative control to OsGSK2-HIS. MBP-CYC U2 was preincubated with OsGSK2-HIS and OsGSK2^K69R-HIS, respectively, and then subjected to the pull-down assay. The top panel is the result of pull-down assay immunoblot with anti-MBP; the bottom and middle panels are the protein loading control. Asterisk indicates the nonspecific binds. e The CYC U2m exhibits a reduced interaction with D3 in GST pull-down assay. Asterisk indicates the nonspecific binds