Fig. 3.

FRET-enhanced mEos3.2 improves tracking of single CHD4 proteins. a Schematic illustrating an experiment where the chromatin remodeler CHD4, a component of the NuRD complex, was fused with a C-terminal mEos3.2-HaloTag in mouse ES cells. b Histograms showing the percentage of molecules with a particular on-state time (i.e., individual track length) remaining after photo-conversion when performing sptPALM of either mEos3.2 (filled bars) or the mEos3.2–JF₆₄₆ FRET pair (open bars) under identical imaging conditions. c Representative 500 ms exposure images from the middle of the nucleus (left) and representative trajectories (right) are shown for mEos3.2- and mEos3.2–JF₆₄₆-tagged single CHD4 molecules (scale bar = 1 µm). d Montages (generated for every 10 frames collected) and plots of the distance moved from their point of photo-conversion, for two mEos3.2- and two mEos3.2–JF₆₄₆-tagged CHD4 molecules