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Percentages of IAHC cells (identified by Gpr56 expression) after different antibody staining and cell sorting strategies
| Sorted cells (staining strategy) | Percentage of cells with Gpr56 transcripts |
|---|---|
| c-kit+ cells (total staining) | 6 |
| c-kit+ cells (intra-aorta staining) | 53 |
| c-kit+CD31− (intra-aorta staining) | 25 |
| c-kit+CD31+ (intra-aorta staining) | 97 |
| c-kit-PE−c-kit-APC+CD31−CD45− (mesenchyme staining) | 0 |
