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. 2018 Jun 28;8:9791. doi: 10.1038/s41598-018-28005-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein quantification of SFTA3 in tissues of the ocular surface as well as tear film and interfacial tension analysis. (A) Quantification of SFTA3 in ocular tissue samples. Mean values of lacrimal gland: 0.712 ng/mg, conjunctiva: 1.908 ng/mg, eyelid: 0.875 ng/mg and in cornea 3.14 ng/mg. (B) Quantification of SFTA3 in tear film. Mean values of healthy subjects: 0.196 ng/mg, EDE: 0.669 ng/mg and in ADDE: 1.157 ng/mg. The protein concentration is expressed in ng/mg and as mean ± SEM. Statistical significance: *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005. (C) Interfacial tension (IFT) for different rhu-SFTA3 concentrations in tear film samples (red). As control we used water (black) and BSA (blue) as protein loading control. The IFT is expressed in mN/m⁻¹ and as mean ± SEM. Statistical significance: *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.