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. 2018 Jun 28;8:9573. doi: 10.1038/s41598-018-27421-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth response of Staphylococcus aureus isolates and coagulase-negative staphylococci (CoNS) to different concentrations of cytokines. Planktonic (panel A) and biofilm (panel B) bacteria were cultured overnight in RPMI medium alone (untreated) and in the presence of different concentrations of IL1-β, IL-6 and IFN-γ. Data are expressed as fold increase (CFU/mL) between treated and untreated cells (dashed red line). The strains were tested in triplicate and experiments were repeated at least three times for each cytokine concentration. Mann-Whitney nonparametric test was used for statistical analysis. *Denotes P < 0.05.