Fig. 6. Electromobility shift assay (EMSA) of rs4693084, rs4693083, and rs4693609 SNPs using allele-specific oligonucleotide probes in various cell lines and primary patient samples.

Nuclear protein extracts from hematological cell lines and AML/ALL primary samples were incubated with three allele-specific biotin-labeled probes. a EMSA for rs4693084, b EMSA for rs4693083, and c EMSA for rs4693609. Note that only rs4693084 SNP affects proteins binding to DNA in the SNP region of both cell lines and primary patient samples. No differences in protein binding between alleles of rs4693083 and rs4693609 SNPs were detected