. 2018 Jun 29;38(3):BSR20171523. doi: 10.1042/BSR20171523

Table 2. Primer sequences for RT-qPCR.

Gene	Primer sequences (5′-3′)
p53	Forward: GACGCTGCCCCCACCATGAG
p53	Reverse: ACCACCACGCTGTGCCGAAA
p21	Forward: GGCAAGAGTGCCTTGACGAT
p21	Reverse: AAGAAAGCCCTCCCCAGTTC
SUMO2	Forward: GGCAACCAATCAACGAAACAG
SUMO2	Reverse: TGCTGGAACACATCAATCGTATC
GADD45α	Forward: TGCTCAGCAAGGCTCGGAGT
GADD45α	Reverse: GTTGCTGACCCGCAGGATGT
CDK2	Forward: CATTCCTCTTCCCCTCATCA
CDK2	Reverse: GTACGGACAGGGACTCCAAA
CDK4	Forward: TTTGATCTCATTGGATTGCC
CDK4	Reverse: AGGTCAGCATTTCCAGCAG
CyclinB1	Forward: CGACAACTGGAGGAAGAGCA
CyclinB1	Reverse: ATGGTCTCCTGAAGCAGCCT
MDM2	Forward: CGGCCTAAAAATGGTTGCAT
MDM2	Reverse: TTTGCACACGTGAAACATGACA
MMP-2	Forward: ACCGTCGCCATCATCAA
MMP-2	Reverse: CCTTCAGCACAAAGAGGTTGC
HIF-1α	Forward: ACAGTGGTACTCACAGTCGG
HIF-1α	Reverse: CCCTGCAGTAGGTTTCTGCT
GAPDH	Forward: CTGGTGCTGAGTATGTCGTGGA
GAPDH	Reverse: AGTTGGTGGTGCAGGATGCATT

Abbreviations: CDK, cyclin-dependent kinases; GADD45α, growth arrest and DNA damage inducible 45α; GAPDH, glyceraldehyde-3-phosphate dehydrogenases; HIF-1α, hypoxia-inducible factor-1α; MDM, mouse double minute; MMP, matrix metalloproteinase; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SUMO2, small ubiquitin-related modifier 2.