Figure 2.

Fore-Paw Pad Abnormalities Are Partly Rescued in the sg1-Cas9-LV-Treated Adult KI-Krt9 Mouse

(A) Fore-paw pads of KI-Krt9 mice treated with Cas9-LV or sg1-Cas9-LV, and those of untreated WT mice. Red arrows indicate injection locations of Cas9-LV and sg1-Cas9-LV. (B) Analysis of epidermal thickness from the pads. (C) Representative images of H&E-stained epidermis showing that melanin (red arrows) and epidermal thickness decreased after sg1-Cas9-LV treatment. Each experiment was repeated at least three times. Scale bars, 100 μm. Mean ± SEM. *p < 0.05; **p < 0.01.