Figure 3.

Correction of the Abnormal IGF1R/IRAIN Production Alters the Phenotypes of Tumor Cells

(A) Cell proliferation. After ALIC targeting, two cell clones were collected for analysis of cell proliferation using the MTT assay. Cell growth was measured as the relative absorbance by setting 0 hour as 1. All experiments were performed in triplicate. The data shown are mean ± SD. (B) Tumor sphere colonies as measured by the soft agar assay. MDA-MB-231 cell colonies were stained on day 15. (C) Quantitation of tumor sphere colonies. Tumor spheres were stained by 0.005% crystal violet and quantitated as the average sphere colonies per microscope field. All data shown are mean ± SD. *p < 0.05, **p < 0.01 as compared with the CTL control. (D) Migration of MBA231 cells. Cell migration was measured by insert plate assay. (E) Quantitation of migrated cells. Cell growth was measured as absorbance at 590 nm. All data shown are mean ± SD. ***p < 0.001 as compared with the CTL control. Note the decreased cell migration in the two targeted cell clones. (F) Cell invasion. Cells that invaded through the collagen-coated membrane of the Transwell were stained with crystal violet 24 hr after cell seeding. (G) Quantitation of invaded cells. Cell growth was measured as absorbance at 590 nm. All data shown are mean ± SD. ***p < 0.001 as compared with the CTL control.