Figure 5.

The Status of DNA Methylation in the IGF1R Promoters

(A) Schematic diagram of the IGF1R/IRAIN gene locus and the location of PCR primers. (B) CpG DNA methylation of site 1. Genomic DNAs were extracted from breast cancer cells. After treated with sodium sulfite, the IRAIN promoter DNA was amplified and sequenced. Open circles, unmethylated CpGs; solid circles, methylated CpGs. All the CpG islands were unmethylated in both the CTL control and ALIC-targeted cells. (C) CpG DNA methylation of site 2. Note the equal DNA hypomethylation in both the CTL control and ALIC-targeted cells.