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. 2018 Jun 22;9:1433. doi: 10.3389/fimmu.2018.01433

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Copyright © 2018 Luo, Olaru, Miner, Beck, van der Vlag, Thurman and Borza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of glomerular immune complexes and complement deposition in B6 mice immunized with α3NC1. Kidneys from adjuvant-immunized B6 mice (left), α3NC1-immunized B6.Cfb^−/− mice (middle), and α3NC1-immunized B6.Cfb^−/− mice (right) were collected at week 12 after the initial immunization. (A) Immunofluorescence staining for mouse IgG shows glomerular basement membrane (GBM) staining in α3NC1-immunized B6.Cfb^−/− and B6.Cfb^+/+ mice. Adjuvant-immunized mice show non-specific mesangial IgG deposition. (B) Mean fluorescence intensity (MFI) of IgG staining, expressed in arbitrary units (AU), was compared in α3NC1-immunized B6.Cfb^−/− and B6.Cfb^+/+ mice. Significance was evaluated by t test (n.s., not significant). (C) Staining with mAb RH34 shows GBM deposition of exogenous antigen in α3NC1 immunized B6.Cfb^−/− and B6.Cfb^+/+ mice, which is absent in control mice. (D) Direct immunofluorescence staining reveals C3c deposition in a capillary loop pattern in α3NC1-immunized B6.Cfb^+/+ mice. C3c staining is absent in B6.Cfb^−/− mice. (E) Indirect immunofluorescence shows C5b-9 deposition along the GBM in α3NC1-immunized B6.Cfb^+/+ mice, but not B6.Cfb^−/− mice. Original magnification 400×.