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. 2018 Jun 22;9:1433. doi: 10.3389/fimmu.2018.01433

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Copyright © 2018 Luo, Olaru, Miner, Beck, van der Vlag, Thurman and Borza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of glomerular immune complexes and complement deposition in D1 mice immunized with α3NC1. Kidneys were collected from adjuvant immunized D1 mice (left), α3NC1-immunized D1.Cfb^−/− mice (middle), and α3NC1-immunized D1.Cfb^−/− mice (right) at week 8 after the initial immunization. (A) Immunofluorescence staining for mouse IgG shows glomerular basement membrane (GBM) staining in α3NC1-immunized D1.Cfb^−/− and D1.Cfb^+/+ mice. Adjuvant-immunized control mice show non-specific mesangial IgG deposition. (B) Mean fluorescence intensity (MFI) of IgG staining, expressed in arbitrary units (AU), was compared in α3NC1-immunized D1.Cfb^−/− and D2.Cfb^+/+ mice. The difference was not significant (n.s.) by t test. (C) Staining with mAb RH34 shows the GBM deposition of exogenous antigen in both groups of α3NC1-immunized mice, which is absent in control mice. (D) Direct immunofluorescence staining reveals capillary loop deposition of C3c in α3NC1-immunized D1.Cfb^+/+ mice, while C3c staining is absent in D1.Cfb^−/− mice. (E) Indirect immunofluorescence shows C5b-9 deposition along the GBM in α3NC1-immunized D1.Cfb^+/+ mice but not D1.Cfb^−/− mice. Original magnification 400×.