Figure 9.

In vitro complement activation by glomerular immune complexes from α3NC1-immunized Cfb^−/− mice. Immunofluorescence analysis of C3c deposition onto kidneys cryosections from α3NC1-immunized D1.Cfb^−/− mice (left) and control non-immunized wild-type DBA/1 mice (right), after incubation with 33% normal mouse serum in buffer containing Ca²⁺ and Mg²⁺ (A), Mg²⁺ and EGTA (B), or EDTA (C). Complement pathways active in each buffer are indicated on the left, according to the key shown at the bottom.