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. 2018 Jun 22;9:1420. doi: 10.3389/fimmu.2018.01420

Figure 4.

Figure 4

DUSP4 expression prevents the DC-SCRIPT-knockdown-mediated change in ERK phosphorylation and IL-10 production. (A) IL-10 protein production by Ctrl-DCs and SC-KD-DCs pre-treated with inhibitors for MEK (upstream of ERK), JNK, p38 or with vehicle control and stimulated with R848 (n = 6, mean + SEM). (B–E) pERK and pp38 phosphorylation levels of Ctrl-DCs and SC-KD-DCs after overexpression of GFP or DUSP4/GFP by phosphoflow (n = 8, mean + SEM). (F) IL-10 protein production by Ctrl-DCs and SC-KD-DCs after overexpression of GFP or DUSP4/GFP (n = 7, mean + SEM). The IL-10 protein data have been normalized to account for donor variation and displayed as arbitrary units (SC-KD-DCs + vehicle range: 0.5–4.6 ng/mL) Statistics: Student’s paired t test. Where no lines are shown, Ctrl-DCs were compared to SC-KD-DCs at the same time point. *p < 0.05, **p < 0.01, ***p < 0.001. FI, fluorescence intensity; AU, arbitrary unit. See also Figure S4 in Supplementary Material.