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. 2018 Jun 22;9:1461. doi: 10.3389/fimmu.2018.01461

Figure 2.

Figure 2

Inducible T-cell co-stimulator (ICOS) promotes development of regulatory T cell (Treg) and T follicular helper (Tfh) cells. Bone marrow transplantation (BMT) was performed as described in Figures 1B–E. 60 days after BMT, recipient spleens were harvested and processed for flow cytometry. Representative contour plots from individual mice (A) and dot plots of mean percentage (B) of Tregs (Foxp3+) on gated donor H-2Kb+CD4 T cells, Tfh cells (PD-1+CXCR5+) on gated H-2Kb+CD4+Foxp3−, and follicular regulatory T (Tfr) cells (PD-1+CXCR5+) on gated H-2Kb+CD4+Foxp3+ T cells are shown, n = 7 mice/group. Representative histogram plots of individual mice from wild-type (WT) group [(C) top] and mean fluorescence intensity (MFI) of ICOS on Tfh and Tfr cells are shown [(C), bottom]. BMT parameters were the same as described in Figures 1B–E, at day 15, 30, 45, and 60 post-BMT, spleens were collected and analyzed by flow cytometry. Summary of percentage of Tregs, Tfh, and Tfr are shown over time (D), n = 3–4 mice/group. *p < 0.05, **p < 0.01, and ***p < 0.001.