Figure 5.

Inducible T-cell co-stimulator (ICOS) on regulatory T cells affects activation of effector T cells. Bone marrow transplantation (BMT) was performed as described in Figure 4. Splenocytes were harvested and processed for flow cytometry 60 days after BMT. Representative dot plots from individual mice and mean percentage of CD44^hiCD62L^lo and CD44^loCD62L^hi on gated H-2K^b+CD4⁺ Foxp3⁻ T cells are shown (A). Representative histogram and mean fluorescence intensity (MFI) of ICOS on gated H-2K^b+CD4⁺Foxp3⁻ T cells are shown (B). n = 4–5 mice/group. Single-cell suspension of splenocytes was processed for intracellular cytokine staining. The representative contour plots from individual mice and mean percentage of IFN-γ⁺, IL-4/5⁺, and IL-17⁺ gated on H-2K^b+CD4⁺ cells are shown (C), n = 4–6 mice/group. *p < 0.05 and **p < 0.01.