Figure 8.
Treatment with anti-inducible T-cell co-stimulator (ICOS) antibodies alleviates chronic graft-versus-host disease. Lethally irradiated BALB/c mice were transferred with 5 × 106 T-cell-depleted bone marrow and 0.5 × 106 SPLs on B6 background. Anti-ICOS Abs were administrated at 200 μg/mouse 3 times/week from day 0 to day 28 after bone marrow transplantation (BMT). Body weight maintenance (A) and clinical score (B) were monitored for 60 days, n = 5 mice/group. Representative pictures of skin and lung stained for collagen 60 days after transplantation are shown (C). Bar graphs of ratios of collagen area to total areas of skin and lung qualified by ImageJ are shown (D). *p < 0.05, **p < 0.01, and ***p < 0.001.