Figure 2.

NKG2A and killer immunoglobulin-like receptors (KIR) subset analysis per cell line. Natural killer (NK) cells were co-cultured with UM9, RPMI8226/s, or JJN-3 cells in a 1:1 E:T ratio for 4 h in 21% O₂ (A) or 0.6% O₂ (B). Flow cytometry was used to subtype NK cells based on their expression of NKG2A and KIRs. Degranulating NK cells were denoted as CD107a+ NK cells. Each dot represents an average of a technical replicate. n = 5 independent experiments with five different donors (UM9), four independent experiments with four different donors (JJN-3), and six independent experiments with six different donors (RPMI8226/s) (ns, not significant, *p < 0.05).