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. 2018 Jun 22;9:1415. doi: 10.3389/fimmu.2018.01415

Figure 3.

Figure 3

Effect of NKG2A expression on natural killer (NK) cells on NK cell activation against multiple myeloma (MM) cell lines and primary MM cells. IL-2-activated NK cells were co-cultured with K562 cell line (A), primary MM (A–D), or without target cells (A) for 4 h in a degranulation assay with or without an NKG2A blocking antibody (C). Flow cytometric analysis was used to subtype NK cells based on their expression of NKG2A and killer immunoglobulin-like receptors (KIR). Degranulating NK cells were denoted as CD107a+ NK cells. Each dot represents an average of a technical replicate. (A–C) n = 10 independent experiments with samples from 10 different MM patients as target cells. (D) n = 2 different myeloma patients used as target cells (ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001).