Figure 5.

Effect of NKG2A and killer immunoglobulin-like receptor (KIR) expression on daratumumab (DARA)-induced natural killer (NK) cell fratricide. NK cells were incubated at 21% O₂ or 0.6% O₂ for 5 h in the absence (A) or presence of DARA (B), or DARA and anti HLA-E (C) or DARA and anti-NKG2A (D). Flow cytometry was used to subtype NK cells based on their expression of NKG2A and KIRs. Degranulating NK cells were denoted as CD107a+ NK cells. Each dot represents the average of a technical replicate (*p < 0.05, **p < 0.01). n = 9 different donors (A,B) or four donors (C,D).