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. 2018 Jun 22;9:1381. doi: 10.3389/fmicb.2018.01381

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Copyright © 2018 Koehler, Cornely, Wisplinghoff, Schauss, Salmanton-Garcia, Ostermann, Ziegler, Bacher, Scheffold, Alex, Richter and Koehler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow Cytometry-Gating strategy and detection of C. glabrata-reactive T cells. Cell frequencies (%). (A) Gating strategy. Negative control, unstimulated CD4⁺ T cells, show no CD69/CD154 expression. (B) Detection of C. glabrata-reactive T cells. From left to right. Negative control; positive control (staphylococcal enterotoxin B stimulated CD4⁺ T cells show CD69/CD154 double-expression); antigen-stimulated probe (CD69/CD154 double expression of C. glabrata-reactive CD4⁺ T cells after antigen stimulation with C. glabrata lysate).