Table 1.

Primers used in this study.

Gene	Oligonucleotide sequence (5′−3′)	Size of product	Annealing temperature	References
nuc-F	GCGATTGATGGTGATACGGTT	798	55	Murakami et al., 1991
nuc-R	AGCCAAGCCTTGACGAACTAAAGC
16S rRNA-F	AGAGTTTGATCATGGCTCAG	270	55
16S rRNA-R	GGACTACCAGGGTATCTAAT
mecA-F	AAAATCGATGGTAAAGGTTGGC	533	55
mecA-R	AGTTCTGCAGTACCGGATTTGC
sea-F	ACGATCAATTTTTACAGC	544	44.5	Rosec and Gigaud, 2002
sea-R	TGCATGTTTTCAGAGTTAATC
seb-F	ATTCTATTAAGGACACTAAGTTAGGGGA	404	44.5	Jarraud et al., 2002
seb-R	ATCCCGTTTCATAAGGCGAGT
sec-F	GACATAAAAGCTAGGAATTT	257	46.2	Rosec and Gigaud, 2002
sec-R	AAATCGGATTAACATTATCCA
sed-F	CAAATATATTGATATAATGA	330	44.5	Khoramrooz et al., 2016
sed-R	AGTAAAAAAGAGTAATGCAA
see-F	CAAAGAAATGCTTTAAGCAATCTTAGGC	482	44.5	Jarraud et al., 2002
see-R	CACCTTACCGCCAAAGCTG
tst-F	ACCCCTGTTCCCTTATCATC	326	54	Khoramrooz et al., 2016
tst-R	TTTTCAGTATTTGTAACGCC
lukS/F-F	ATCATTAGGTAAAATGTCTGGACATGATCCA	433	55	McClure et al., 2006
lukS/F-R	GCATCAAGTGTATTGGATAGCAAAAGC
pan-agr	ATGCACATGGTGCACATGC	–	55	Shopsin et al., 2003
agrI	GTCACAAGTACTATAAGCTGCGAT	440	55
agrII	GTATTACTAATTGAAAAGTGCCATAGC	573	55
agrIII	CTGTTGAAAAAGTCAACTAAAAGCTC	406	55
agrIV	CGATAATGCCGTAATAC CCG	588	55
fnbA-F	GATACAAACCCAGGTGGTGG	191	52	Zmantar et al., 2008
fnbA-R	TGTGCTTGACCATGCTCTTC
fnbB-F	ACGCTCAAGGCGACGGCAAAG	197	62	Pereyra et al., 2016
fnbB-R	ACCTTCTGCATGACCTTCTGCACCT
clfA-F	CCGGATCCGTAGCTGCAGATGCACC	1000	60	Zmantar et al., 2008
clfA-R	GCTCTAGATCACTCATCAGGTTGTTCAGG
clfB-F	TGCAAGTGCAGATTCCGAAAAAAAC	194	62	Klein et al., 2012
clfB-R	CCGTCGGTTGAGGTGTTTCATTTG
cna-F	AAAGCGTTGCCTAGTGGAGAC	192	54	Zmantar et al., 2008
cna-R	AGTGCCTTCCCAAACCTTTT
bap-F	CCCTATATCGAAGGTGTAGAATTG	971	60	Darwish and Asfour, 2013
bap-R	GCTGTTGAAGTTAATACTGTACCTGC
icaA-F	CCTAACTAACGAAAGGTAG	1351	49
icaA-R	AAGATATAGCGATAAGTGC
icaD-F	AAACGTAAGAGAGGTGG	381	49	Pereyra et al., 2016
icaD-R	GGCAATATGATCAAGATAC