Table 5.
Phenotypes and genotypes of 96 S. aureus isolates tested in this study.
| Phenotypes or genotypes tested in this study | No. of isolates (%) | |||
|---|---|---|---|---|
| Farm A | Farm B | Total | ||
| Antimicrobial resistance patterns | PEN | 3(4.1) | 13(56.5)* | 16(16.7) |
| CIP | 1(1.4) | 0(0) | 1(1) | |
| ENO | 1(1.4) | 0(0) | 1(1) | |
| TET | 1(1.4) | 0(0) | 1(1) | |
| PEN-CIP | 3(4.1) | 0(0) | 3(3.1) | |
| CIP-ENO | 0(0) | 4(17.4) | 4(4.2) | |
| PEN-CIP-ENO | 5(6.8) | 0(0) | 5(5.2) | |
| PEN-CHL-GEN-TIL | 1(1.4) | 0(0) | 1(1) | |
| PEN-CIP-ENO-ERY-TIL | 1(1.4) | 4(17.4)* | 5(5.2) | |
| ND | 57(78.1) | 2(8.7) | 59(61.5) | |
| Virulence genes | tst | 2(2.7) | 0(0) | 2(2.1) |
| pvl | 71(97.3)* | 19(82.6) | 90(93.8) | |
| sea | 58(79.5) | 0(0) | 58(60.4) | |
| seb | 0(0) | 14(60.9) | 14(14.6) | |
| sec | 62(84.9)* | 1(4.3) | 63(65.6) | |
| sed | 4(5.5) | 1(4.3) | 5(5.2) | |
| see | 0(0) | 0(0) | 0(0) | |
| ND | 1(1.4) | 4(17.4) | 5(5.2) | |
| Virulence gene patterns | pvl | 10(13.7) | 4(17.4) | 14(14.6) |
| pvl-seb | 0(0) | 13(56.5) | 13(13.5) | |
| pvl-sec | 0(0) | 1(4.3) | 1(1) | |
| sea-sec | 1(1.4) | 0(0) | 1(1) | |
| pvl-sea-sec | 57(78.1) | 0(0) | 57(59.4) | |
| pvl-seb-sed | 0(0) | 1(4.3) | 1(1) | |
| pvl-sec-sed | 2(2.7) | 0(0) | 2(2.1) | |
| tst-pvl-sec-sed | 2(2.7) | 0(0) | 2(2.1) | |
| ND | 1(1.4) | 4(17.4) | 5(5.2) | |
| Enterotoxin production | SEA | 53(72.6) | 0(0) | 53(55.2) |
| SEB | 0(0) | 14(60.9) | 14(14.6) | |
| SEC | 58(79.5)* | 1(4.3) | 59(61.5) | |
| SED | 4(5.5) | 1(4.3) | 5(5.2) | |
| SEE | 0(0) | 0(0) | 0(0) | |
| ND | 11(15.1) | 8(34.8) | 19(19.8) | |
| Enterotoxin production patterns | SEA | 4(5.5) | 0(0) | 4(4.2) |
| SEB | 0(0) | 13(56.5) | 13(13.5) | |
| SEC | 5(6.8) | 1(4.3) | 6(6.3) | |
| SEA-SEC | 49(67.1) | 0(0) | 49(51) | |
| SEB-SED | 0(0) | 1(4.3) | 1(1) | |
| SEC-SED | 4(5.5) | 0(0) | 4(4.2) | |
| ND | 11(15.1) | 8(34.8) | 19(19.8) | |
| Biofilm related genes | icaA-icaD-clfA-clfB-can-fnbA | 5(6.8) | 2(8.6) | 7(7.3) |
| icaA-icaD-clfA-clfB-can-fnbA-fnbB | 68(93.2) | 20(86.9) | 88(91.7) | |
| bap-icaA-icaD-clfA-clfB-can-fnbA-fnbB | 0(0) | 1(4.3) | 1(1) | |
| Biofilm production abilitya | + (range of OD: 0.913-1.196) | 4(5.5) | 4(17.39) | 8(8.3) |
| ++ (range of OD: 1.246-1.797) | 53(72.6) | 17(73.9) | 70(72.9) | |
| +++ (range of OD: 1.807-2.156) | 16(21.9) | 2(8.7) | 18(18.8) | |
| agr types | I | 6(8.2) | 8(34.8) | 14(14.6) |
| II | 0(0) | 0(0) | 0(0) | |
| III | 54(74) | 15(65.2) | 69(71.9) | |
| IV | 13(17.8) | 0(0) | 13(13.5) | |
| MLST | CC1-ST1 | 54(74) | 15(65.2) | 69(71.9) |
| CC7-ST7 | 0(0) | 4(17.4) | 4(4.2) | |
| CC50-ST50 | 13(17.8) | 0(0) | 13(13.5) | |
| CC97-ST97 | 4(5.5) | 0(0) | 4(4.2) | |
| CC398-ST398 | 2(2.7) | 4(17.4) | 6(6.3) | |
| spa typing | t034 | 1(1.4) | 0(0) | 1(1) |
| t127 | 54(74) | 0(0) | 54(56.3) | |
| t518 | 13(17.8) | 0(0) | 13(13.5) | |
| t730 | 4(5.5) | 0(0) | 4(4.2) | |
| t2279 | 0(0) | 15(65.2) | 15(15.6) | |
| t14156 | 1(1.4) | 4(17.4) | 5(5.2) | |
| t17182 | 0(0) | 4(17.4) | 4(4.2) | |
| Genotype patterns | PFGE cluster I-CC1-ST1-t2279-agr III | 0(0) | 15(65.2) | 15(15.6) |
| PFGE cluster II-CC1-ST1-t127-agr III | 54(74) | 0(0) | 54(56.3) | |
| PFGE cluster III-CC97-ST97-t730-agr I | 4(5.5) | 0(0) | 4(4.2) | |
| PFGE cluster IV-CC7-ST7-t17182-agr I | 0(0) | 4(17.4) | 4(4.2) | |
| PFGE cluster V-CC50-ST50-t518-agr IV | 13(17.8) | 0(0) | 13(13.5) | |
| PFGE cluster VI-CC398-ST398-t034-agr I | 1(1.4) | 0(0) | 1(1) | |
| PFGE cluster VI-CC398-ST398-t14156-agr I | 1(1.4) | 4(17.4) | 5(5.2) | |
a
Quantification of biofilm formation by optical density (OD) determination: (+++): strong biofilm producers (OD570 > 1.8), (++): moderate biofilm producers (1.8 > OD570 > 1.2), (+): weak biofilm producers (1.2 > OD570 > 0.6);
*
p < 0.05.