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. 2018 Jun 22;9:747. doi: 10.3389/fphys.2018.00747

FIGURE 4.

FIGURE 4

Characterization of cell metabolism during stem cell differentiation into cardiomyocytes using the extracellular flux analyzer. (A) Determination of the energetic profile of mESC (open circles) and mESC-derived CM at d14 (gray filled circles) using extracellular flux analyzer under standard media conditions from three independent experiments. (B,C). Quantitative RT-PCR mRNA transcript analysis of β-oxidation markers (PGC1a, FATP6, CPT1B, and ACADVL) at different time points during mouse heart development (B) and mESC differentiation into cardiomyocytes (C). Results are presented relative to mRNA amounts of the housekeeping gene GAPDH. Each data point represents the mean value ± SEM of at least n = 3 animals per age.