Table 1.

Effect of duration of enzyme digestion on protoplast yield and viability.

Digestion duration in enzyme solution supplemented with 0.7 M mannitol	Protoplast Yield (×105 cells/ petal)	Viability (%)
8 h	2.2 ± 0.4	89.1 ± 3.3
16 h	6.1 ± 0.5	90.1 ± 2.4

^∗Standard deviations were derived from three independent experiments.