Table 2.

Effect of mannitol concentration on protoplast viability during the isolation procedure.

After enzyme digestion and resuspension in WI based buffer	Viability (%)
0.4 M	90.7 ± 5.7
0.6 M	91.6 ± 4.5
0.7 M	91.1 ± 6.1
0.8 M	94.1 ± 2.1
After washing and resuspension in MMG based buffer	Viability (%)
0.4 M	77.3 ± 10.5
0.6 M	81.1 ± 8.2
0.7 M	81.3 ± 0.4
0.8 M	73.9 ± 9.2
After transfection	Viability (%)
0.4 M	66.6 ± 2.6
0.6 M	75.3 ± 4.2
0.7 M	77.5 ± 2.4
0.8 M	67.9 ± 12.4

^∗Standard deviations were derived from at least three independent experiments.