Table 4: Performance of the different primer suppliers (CIGB and BCD) on the Roche LightCycler® 480 II

		Mean Cp				SD				CV (%)
Concentration		CIGB		BCD		CIGB		BCD		CIGB		BCD
900,000 c/r		15.55		15.59		0.05		0.07		0.34		0.42
90,000 c/r		18.28		18.43		0.05		0.14		0.28		0.75
9,000 c/r		21.93		21.89		0.06		0.03		0.28		0.14
900 c/r		25.2		25.14		0.12		0.14		0.46		0.55
90 c/r		27.81		27.94		0.08		0.14		0.29		0.51
9 c/r		29.62		29.51		0.12		0.27		0.39		0.92
0.9 c/r		29.96		29.96		0.20		0.04		0.65		0.13
0.09 c/r		30.96		30.55		0.49		0.04		1.58		0.14

Dilution series with the pST012012 plasmid at concentrations of 900,000 to 0.09 c/r were used, and three replicates per dilution step were measured in the qPCR. The means of Ct or Cp values were compared between the thermocyclers. SD: Standard deviation; CV (%): Coefficient of variation expressed in percent