Figure 5.

Effect of Mutations in the PB2 627 and NLS Domains on FluPol_C Function

The effect of single-amino-acid mutations in the PB2 627 and NLS domains proximal to CTD-binding site 1 was analyzed by RNP reconstitution assays. vRNA, mRNA, and cRNA levels were analyzed by primer extension and quantitated by phosphorimage analysis with 5S rRNA as a loading control. RNA levels generated by the WT polymerase were set to 100%. RNP reconstitutions without PB2 (−PB2) served as negative controls. The mean of five independent experiments is shown with error bars representing SD. Asterisks indicate a significant difference from WT (two-tailed one-sample t test) as follows: ^∗p < 0.05 and ^∗∗p < 0.01.

See also Figure S5.