Figure 6.

Effect of Pol II CTD Peptides on FluPol_C Endonuclease and Transcriptional Activity

In vitro endonuclease and cap-dependent transcription initiation assays were performed using purified FluPol_C in the presence of pS₅, pS₂, un-phosphorylated (UnP), or scrambled (Scr) Pol II CTD peptides. Assays were carried out in the absence or presence of 5′ and 3′ vRNA promoter RNAs. The absence or presence of rNTP substrates is indicated. Top panel: capped RNA cleavage assay is shown; middle panel: capped RNA cleavage and transcription initiation assay are shown; bottom panel: cap-dependent transcription initiation assay is shown. The position of a non-specific cleavage product that partially overlaps with the viral polymerase-specific cleavage products is indicated by a star (^∗).

See also Figure S6.