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. 2018 Jun 22;8:208. doi: 10.3389/fcimb.2018.00208

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Copyright © 2018 Li, Huang, Cui, Liu, Hou, Zhang, Liu, Meng and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm formation of the ΔtolC strain under hyper-osmotic conditions was restored by overproducing OmpX. Cells of each indicated strain were inoculated from overnight grown culture into 1/2 M9 (A), M9 (B), 1/2M9 supplemented with 0.8% sucrose (C), and 1/2 M9 supplemented with 0.06 M NaCl (D). The cells were collected after 120 h growth at 28°C and the biofilms were measured using crystal violet biofilm assay. The data shown are the mean of 12 biological replicates. The cutoff OD (ODc) was defined as three times standard deviation above the mean OD₆₃₀ value of the negative control.