Table 1.
Strains, plasmids, and primers used in this work.
| Description or sequence | Source | |
|---|---|---|
| STRAIN | ||
| ExPEC strain PPECC42 | Wild-type (WT), porcine origin, CmS | Hou et al., 2014 |
| ΔtolC | Mutant with a 158-bp fragment deleted from the whole ORF of the tolC gene in PPECC42, CmS | Hou et al., 2014 |
| ΔompX | Mutant with a 516-bp fragment deleted from the whole ORF of the ompX gene in PPECC42, CmS | Meng et al., 2016 |
| ΔtolC::ompX | ΔtolC mutant carrying plasmid pHSG::ompX, CmR | This study |
| WT::ompX | PPECC42 strain carrying plasmid pHSG::ompX, CmR | This study |
| χ7213 | Thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44ΔasdA4 recA1 RP4 2-Tc::Mu[λpir] KmR | Hou et al., 2014 |
| DH5α | F–,ϕ80dlacZΔM15, Δ(lacZYA-argF) U169,deoR, recA1, endA1, hsdR17 (rk−,mk+), phoA,supE44, λ−,thi-1,gyrA96,relA1 | Takara Bio |
| PLASMID | ||
| pRE112 | oriT oriVΔasdCmRSacB, suicide vector | Hou et al., 2014 |
| pHSG | ori lacZ CmR pHSG396 | Takara Bio |
| pHSG::ompX | ori lacZ CmR pHSG396 with the full-length ompX gene | Meng et al., 2016 |
| PRIMER | ||
| ompXF | ACCTGAAATACCGCTATGAA | |
| ompXR | TCAGTGGTCTGGAATTTACC | |
| csgDF | CCCGTACCGCGACATTG | |
| csgDR | ACGTTCTTGATCCTCCATGGA | |
| csgBF | CATAATTGGTCAAGCTGGGACTAA | |
| csgBR | GCAACAACCGCCAAAAGTTT | |
| GAPDHF | ACTTACGAGCAGATCAAAGC | |
| GAPDHR | AGTTTCACGAAGTTGTCGTT | |