FIG 3.

Hematopoietic miR-33 deficiency increased LSK, LK cells, and Ly6C^high monocytes in BM, and nonhematopoietic miR-33 deficiency reduced peripheral Ly6C^high monocytes. (A) Experimental protocol for BMT. w, weeks. (B) The proportion of Ly6C^high monocytes in peripheral monocytes (left) and the proportion (middle) and the number (right) of Ly6C^high monocytes in PB from four groups of mice (n = 12 to 19 per group). (C) The proportion (left) and the number (right) of Ly6C^high monocytes in BM from four groups of mice (n = 10 to 12 per group). (D) The proportion and the number of LSK (upper left and right) and LK (lower left and right) cells in BM from four groups of mice (n = 26 to 29 per group). (E) The proportion of apoptosis in LSK cells from miR-33^+/+ recipient mice transplanted with miR-33^+/+ or miR-33^−/− BM (miR-33^+/+, n = 12 BM; miR-33^−/−, n = 10 BM). All data are shown as means ± SEM. *, P < 0.05; **, P < 0.01, ***, P < 0.001 (by 1-way ANOVA with the Turkey post hoc test for panels A to D); *, P < 0.05 (by Student's t test for panel E).