FIG 4.

Hematopoietic miR-33 deficiency increases peripheral Ly6C^high monocytes without nonhematopoietic miR-33 deficiency. (A) Experimental protocol for the competitive repopulation assay. (B) The percentage of CD45.2⁺ leukocytes, Ly6C^high monocytes, and CD11b⁺ myeloid cells, as indicated, in PB from CD45.1⁺ miR-33^+/+ mice transplanted with equally mixed CD45.1⁺ miR-33^+/+ and CD45.2⁺ miR-33^+/+ (miR-33^+/+ group) or CD45.2⁺ miR-33^−/− BM (miR-33^−/− group) cells (miR-33^+/+ group, n = 5; miR-33^−/− group, n = 14). (C) The percentage of CD45.2⁺ LSK and LK cells in the miR-33^+/+ group and the miR-33^−/− group (miR-33^+/+ group, n = 5;, miR-33^−/− group, n = 14). All data are shown as means ± SEM. *, P < 0.05; **, P < 0.01 (by Mann-Whitney U test).