FIG 2.

TOR facilitates formation of the preinitiation complex (PIC) at the RPS5 promoter to enhance transcription. (A) Inhibition of TOR by rapamycin decreases the recruitment of TBP, TAF1p, and Rpb1p to the core promoter of RPS5. Yeast cells were grown as for Fig. 1A. (B) Reverse transcription-PCR (RT-PCR) analysis. Rapamycin inhibition of TOR impairs transcription of the RPS5 gene. (C) Inhibition of TOR by rapamycin does not impair the recruitment of TBP and Rpb1p to the ACT1 promoter. (D) Growth analysis of rpt4-ts (or sug2-ts) and wild-type (WT) strains in liquid YPD growth medium at the nonpermissive temperature (37°C). Yeast cells were initially grown at 23°C up to an OD₆₀₀ of about 0.1 prior to switching temperature to 37°C, and then OD₆₀₀ was measured at different time points for growth analysis. (E and F) RT-PCR analysis of RPS5, TSR2, and MGP in HEK293 cells following rapamycin inhibition of TOR.