FIG 3.

TOR facilitates targeting of the 19S proteasome subcomplex to the UASs of RPL2B and RPS11B for enhanced recruitment of NuA4 KAT for histone H4 acetylation. (A) TOR facilitates the targeting of the 19S proteasome subcomplex to the UASs of RPL2B and RPS11B. A yeast strain expressing Myc-tagged Rpt6p was grown, cross-linked, and immunoprecipitated as for Fig. 1A. (B and C) Rapamycin inhibition of TOR does not alter the recruitment of activator Rap1p to the UASs of RPL2B and RPS11B but rather reduces the targeting of NuA4 KAT. (D and E) Histone H4 acetylation at the RPL2B and RPS11B promoters is impaired following rapamycin inhibition of TOR.