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NuA4 KAT is dispensable for recruitment of the 19S proteasome subcomplex to the RPS5, RPL2B, and RPS11B promoters. (A to C) ChIP analysis of Rpt2p at the UASs of RPS5, RPL2B, and RPS11B. Yeast cells expressing Myc-tagged Rpt2p were grown in YPD up to an OD600 of 0.9 and then switched to 37°C for 1 h prior to cross-linking. (D) Growth analysis of wild-type, eaf1Δ, sug2-ts (or rpt4-ts), and eaf1Δsug2-ts (or eaf1Δrpt4-ts) strains in the solid YPD medium at 23°C, 30°C, and 37°C.
