FIG 8.

Schematic diagram showing TOR-mediated RP gene activation. Rap1p interacts with the 19S proteasome subcomplex (19S regulatory particle [RP]) and recruits it to the UAS of the RP gene. The 19S RP subsequently promotes the targeting of NuA4 to the activator Rap1p. NuA4 facilitates recruitment of TFIID (transcription factor IID, which is composed of TBP and a set of TBP-associated factors [TAFs]) toward the formation of the PIC, which includes RNA polymerase II for transcriptional initiation. TOR promotes the targeting of the 19S RP to the activator and hence facilitates recruitment of NuA4 and subsequent PIC formation and transcriptional initiation. ORF, open reading frame. +, stimulation.