Fig. 3. Confocal fluorescence images of live HepG2 cells stained simultaneously with ER-H₂O₂ and MI-H₂O₂ (10 μM). ER-H₂O₂ was excited by 405 nm, and collected at 500–620 nm for the green channel (image A) and at 430–470 nm for the blue channel (image B). MI-H₂O₂ was excited by 543 nm, and collected at 550–600 nm for the red channel (image C). (D) was the bright-field image. (E) was overlay of (A) and (C). (F) was the enlarged image from the square marked in image (E). Scare bar: 20 μm.