Fig. 7. Binding of compound 1 to RocR. (A) Normalized RocR intrinsic fluorescence change with different concentrations of compound 1, indicating a dissociation constant Kd of 14 ± 2 μM (eqn (1)†).47 (B) Stern–Volmer plot of the RocR intrinsic fluorescence. Kd was calculated as 16 ± 2 μM using eqn (2),48 see Methods section.† .
