Fig. 5. Cell labelling with Cu(i)/M-dots for fluorescence microscopy imaging. The scale bar represents 40 µm. (A) U87MG cells were first treated with RGD-alkyne (10 µM) for 30 min at 37 °C, followed by incubation with Cy5.5 azide (30 µM) and 4 µM Cu(i)/M-dots (containing 200 µM Cu+) for 60 min at 37 °C. After cells were fixed with 4% PFA, DAPI staining was performed. (B) Control experiment without adding Cu(i)/M-dots for incubation. (C) Control experiment without adding Cy5.5 azide for incubation.
