Figure 2.

Down-selected antibodies neutralizing activity was evaluated by PRNT. Serially diluted mAbs were mixed in complete MEM supplemented with 10% FBS with SUDV at a constant viral titer of 65 pfu prior to adding in to Vero cells for a 1 h incubation. Wells were overlaid with 1% agarose in Eagle’s basal medium (EBME) with 10% FBS and 0.1% gentamicin and returned to the incubator On day 7, a 1% agarose secondary overlay containing 4% neutral red was added and after one more day at 37 °C, plaques were counted.