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. 2018 Jun 19;10(6):250. doi: 10.3390/toxins10060250

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Protein sequences of two toxins identified in Tb II-I. This fraction was submitted to in solution digestion by (A) trypsin, (B) chymotrypsin or (C) formic acid, and the peptidomic analysis was performed against a transcriptome database obtained from the venom gland of Tityus bahiensis. The identified peptides for each protein are highlighted. Two beta sodium toxins Tbah02791 (known as Toxin Tb4; UniProt code: P56610) and Tbah02765 (known as Toxin Tb2 II; UniProt code: P60276) were identified. (D) Tbah02791 and Tbah02765 Clustal O (1.2.4) sequence alignment analysis, showing that both sequences have 56.72% identity.