Figure 3.

Interaction between PNRC2 and nuclear receptors in yeast. Yeast strain Y187 was co-transformed with the appropriate pGBT9-receptor and pACT2-PNRC2 constructs and transformants harboring both plasmids were selected by growth on SD/–Leu/–Trp agar plates. The expression of interacting hybrid proteins in Y187 transformants was analyzed for induction of LacZ expression in the presence of the indicated amount of ligands as described in Materials and Methods. Gal4 AD was included as a control to monitor background transcriptional activity. Relative β-galactosidase activities in liquid cultures are expressed in Miller units as means ± SD of three independent assays.