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. 2001 Oct 1;29(19):3939–3948. doi: 10.1093/nar/29.19.3939

Figure 5.

Figure 5

Figure 5

Coactivation functional analysis of PNRC2 on transcription mediated by multiple nuclear receptors in HeLa cells. HeLa cells were transfected with 0.25 µg pGL3(SF1 site)3-Luciferase reporter plasmid along with 10 ng expression plasmid for SF1 (pSG5-SF1) (A and D) or for ERRα1 (pSG5-hERRα1) (B) and increasing amounts of pSG5-PNRC2 expression plasmid as indicated. Appropriate amounts of empty vector pSG5 were included to maintain the 1.25 µg total DNA in all transfections. The luciferase activities in transfected cells were measured and are expressed as means ± SD of at least triplicate experiments. (A) Effect of PNRC2 overexpression on SF1-stimulated transcription of the luciferase gene directed by the SV40 promoter. (B) Effect of PNRC2 overexpression on ERRα1-stimulated transcription of the luciferase gene directed by the SV40 promoter. (C) Effect of PNRC2 overexpression on transcription mediated by human ERα. HeLa cells were transfected with pGL3(ERE)3-Luciferase reporter plasmid along with either pSG5-hERα expression plasmid alone or together with increasing amounts of PNRC2 expression plasmid (pSG5-PNRC2). All other features are as described for (A) and (B). (D) Comparison of coactivator activities of PNRC and PNRC2. The procedures for transfection and luciferase assay were the same as described for (A).