Figure 7.

The C-terminal SH3-binding motif-containing domain of PNRC functions as a dominant negative inhibitor in the coactivation function of PNRC2. Expression plasmids (1.0 µg each) for wild-type PNRC2 (pSG5-PNRC2) and the C-terminal 57 amino acids of PNRC in the sense [pSG5-PNRC_{270–327 (S)}] or antisense [pSG5-PNRC_{270–327 (A)}] orientation along with 0.2 µg reporter plasmid [pGL₃(SF1 site)₃-Luciferase] and 10 ng SF1 expression plasmid (pSG5-SF1) were co-transfected into HeLa cells. The luciferase activities in the cell lysates from the transfected cells were assayed. Each result represents the mean ± SD of four experiments.