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. 2016 Jun;2(2):79–84. doi: 10.18383/j.tom.2016.00151

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© 2016 The Authors. Published by Grapho Publications, LLC

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Figure 5. — Isolation of live green fluorescent protein (GFP)+ Flash Red+ cells from whole mouse brain. Perl's Prussian Blue staining (blue) confirmed MPIO labeling of 231BR cells; 10× magnification (A). MRI confirmed the successful delivery of MPIO-labeled 231BR cells to mouse brain (arrows) (B). FACS analysis of dissociated mouse brain concentrated with a magnetic column identified and isolated a subset of live GFP+ Flash Red+ cells from the dissociated brain cell population. GFP+ Flash Red+ cells were only found and isolated from mice that had received labeled 231BR cell injections (C).