Table 2.

The effects of Fyn kinase on the gating properties of Nav1.7 channels transiently transfected in HEK 293 and ND7/23 cells.

	Activation			Steady-state fast inactivation			Steady-state slow inactivation
	V1/2,act	k	n	V1/2,fast	k	n	V1/2, slow	k	Rresist (%)	n
Nav1.7r-WT, HEK 293
+ FynDN	−20.6 ± 0.6	7.18 ± 0.15	16	−74.0 ± 0.4	5.45 ± 0.13	16	−63.1 ± 1.3	10.4 ± 0.4	12.6 ± 1.0	10
+ FynCA	−17.2 ± 0.6*	7.34 ± 0.19	12	−76.7 ± 0.9*	7.26 ± 0.22*	12	−62.9 ± 1.6	11.9 ± 0.2*	11.0 ± 0.9	8
Nav1.7rG-WT, ND7/23
+ FynDN	−15.2 ± 0.9	7.56 ± 0.19	14	−74.7 ± 1.4	6.56 ± 0.20	13	−59.1 ± 0.7	11.2 ± 0.4	22.9 ± 1.8	12
+ FynCA	−20.4 ± 2.1*	7.62 ± 0.40	11	−82.8 ± 2.6*	6.81 ± 0.31	11	−65.9 ± 1.8*	11.4 ± 0.7	16.4 ± 2.5*	9
Nav1.7rG-YYFF, ND7/23
+ FynDN	−15.0 ± 1.3	8.37 ± 0.25	10	−74.5± 1.8	7.99 ± 0.34#	10	−60.9 ± 1.9	10.0 ± 0.5	16.6 ± 2.0#	9
+ FynCA	−19.1 ± 1.2* #	8.10 ± 0.33	15	−82.4 ± 1.4* #	8.69 ± 0.21#	13	−64.2 ± 0.9#	11.0 ± 0.3	12.0 ± 1.1#	14

Note: * p < 0.05 Fyn^CA vs corresponding Fyn^DNcontrol, two-sample student t test (HEK 293 cells) or one-way ANOVA test (ND7/23 cells); ^# p < 0.05 Nav1.7rG-YYFF/Fyn^DN or Nav1.7rG-YYFF/Fyn^CA vs Nav1.7rG-WT/Fyn^DN, one-way ANOVA test (ND7/23 cells).